| Person | [Birth - Death] | Spouse |
|---|---|---|
| Middleton | [? - ?] | |
| Achsah Middleton | [about 1740 - 1801] | |
| Anthony Middleton | [? - before 12 April 1592] | |
| Ardon Plimpton Middleton | [21 February 1833 - 19 July 1911] | Caroline Hutchinson |
| Asa Middleton | [about 1739 - 1766] | |
| Cynthia Middleton | [? - ?] | Micajah K Pickett |
| Elizabeth Middleton | [? - ?] | |
| Elizabeth Middleton | [? - (27 July 1740-18 November 1740)] | |
| Gabriel Middleton | [? - after 12 April 1592] | |
| George Middleton | [? - ?] | Dinah |
| George Middleton | [12 July 1722 - (1743-1777)] | Hannah Fowler |
| Hugh Middleton | [? - ?] | |
| Jane Middleton | [? - after 1740] | John Richardson |
| Jasper Middleton | [? - after 12 April 1592] | |
| Joel Middleton | [about 1741 - after 1765] | |
| John Middleton | [? - before 1710] | Martha |
| John Middleton | [1686 - 25 March 1741] | Esther Gilberthorpe |
| John Middleton | [22 November 1712 - 3 February 1765] | Sarah Ivins |
| John Middleton | [about 1742 - 1804] | |
| Jonathan Middleton | [about 1744 - 1783] | |
| Joseph Middleton | [? - after 1794] | Avis Holloway |
| Martha Middleton | [1686 - 1767] | John Rogers |
| Mary Middleton | [? - after 1712] | |
| Miriam Middleton | [10 February 1766 - (1796-1799)] | Aaron Ivins |
| Naomi Middleton | [? - ?] | |
| Nathan Middleton | [? - ?] | |
| Nathan Middleton | [? - ?] | |
| Nathan Middleton | [? - (6 June 1760-30 June 1760)] | Mary French |
| Nathan Middleton | [? - ?] | Judith Stokes |
| Nicholas Middleton | [? - after 12 April 1592] | |
| Thomas Middleton | [? - 12 April 1592] | Amy |
| Thomas Middleton | [? - 1704] | |
| Thomas Middleton | [? - ?] | |
| Thomas Middleton | [? - after 1712] | |
| Thomas Middleton | [? - ?] | Mercy Allen |
| Ursula Middleton | [? - about 30 October 1596] | John Roote |
| William Middleton | [? - after 12 April 1592] | |
| William Middleton | [? - after 1712] |