Staining with Iodine



The iodine staining technique allows us to carry around a marked version of our TLC run rather than having to pencil sketch our spots in the UV viewer. The iodine vapors chemically attach to analytes on the TLC plate. While this makes the samples unrecoverable for future use (usually you will not need to recover your sample from a TLC plate), it makes analysis easier. Some compounds might not even appear under UV light, making other visualization techniques such as iodine staining necessary.