1. W. Cheng. (2016). The Density Code for the Development of a Vaccine? J. Pharm. Sci. doi: 10.1016/j.xphs.2016.07.020. [Abstract][PDF]
  2. M. C. DeSantis, and W. Cheng. (2016). Label-free Detection and Manipulation of Single Biological Nanoparticles. WIRES Nanomedicine and Nanobiotechnology doi: 10.1002/wnan.1392. [Abstract][PDF]
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  4. W. Cheng. (2012). Force-fluorescence spectroscopy. In: Roberts GC. Encyclopedia of Biophysics. Heidelberg, Springer. [PDF]
  5. X. Hou, and W. Cheng. (2012). Optical Tweezers. In: Roberts GC. Encyclopedia of Biophysics. Heidelberg, Springer. [PDF]
  6. C. Bustamante, W. Cheng, and Y. X. Meija. (2011). Revisiting the Central Dogma One Molecule at a Time. Cell. 144: 480-497. [ Abstract ] [ PDF ]

Research Articles

  1. Wei-Yun Wholey, Sekou-Tidiane Yoda and W. Cheng. (2021) Site-Specific and Stable Conjugation of the SARS-CoV-2 Receptor-Binding Domain to Liposomes in the Absence of Any Other Adjuvants Elicits Potent Neutralizing Antibodies in BALB/c Mice. Bioconjugate Chem. [Link to article]. Selected as ACS Editors' Choice.   
  2. M. C. DeSantis, C. Tian, J. H. Kim, J. L. Austin and W. Cheng. (2020) Probability of Immobilization on Host Cell Surface Regulates Viral Infectivity. Phys. Rev. Lett. 125: 128101 [Abstract][PDF].
  3. Wei-Yun Wholey, J. L. Mueller, C. Tan, J. F. Brooks, J. Zikherman and W. Cheng. (2020) Synthetic Liposomal Mimics of Biological Viruses for the Study of Immune Responses to Infection and Vaccination. Bioconjugate Chem. [Abstract][PDF].
  4. Z. Chen, Wei-Yun Wholey, A. Hassani Najafabadi, J. J. MoonI. GrigorovaB. Chackerian and W. Cheng. (2019) Self-Antigens Displayed on Liposomal Nanoparticles above a Threshold of Epitope Density Elicit Class-Switched Autoreactive Antibodies Independent of T Cell Help. J. Immunol. December 13, 2019, ji1801677; DOI: [Abstract][PDF].
  5. A. Kotnala, Y. Zheng, J. Fu, and W. Cheng. (2019) Back-focal-plane interferometric detection of nanoparticles in spatially confined microfluidic channels. Rev. Sci. Instrum. 90: 023107. doi: 10.1063/1.5074194. [Abstract][PDF].
  6. K. I. Schimert, and W. Cheng. (2018). A method for tethering single viral particles for virus-cell interaction studies with optical tweezers. Proc. of SPIE 10723: 10723B. doi: 10.1117/12.2500050. [Abstract][PDF].
  7. H. Song, M. C. DeSantis, C. Tian, and W. Cheng (2018) Dynasore inhibition on productive infection of HIV-1 in commonly used cell lines is independent of transferrin endocytosis. Science Matters doi: 10.19185/matters.201805000001. [PDF].
  8. Z. Chen, JJ Moon, and W. Cheng. (2018) Quantitation and stability of protein conjugation on liposomes for controlled density of surface epitopes. Bioconjugate Chem. doi: 10.1021/acs.bioconjchem.8b00033. [Abstract][PDF].
  9. B. Hua, Y. Wang, S. Park, KY Han, D. Singh, JH Jim, W. Cheng, and T. Ha. (2018) The single-molecule centroid localization algorithm improves the accuracy of fluorescence binding assays. Biochemistry 57: 1572-1576. [Abstract][PDF].
  10. A. Kotnala, Y. Zheng, J. Fu, and W. Cheng. (2017) Microfluidic-based high-throughput optical trapping of nanoparticles. Lab Chip. 17: 2125-2134. doi: 10.1039/C7LC00286F. [Abstract][PDF].
  11. Z. Chen, and W. Cheng. (2017). Reversible aggregation of HIV-1 Gag proteins mediated by nucleic acids. Biochem. Biophys. Res. Comm. 482: 1437-1442. [Abstract][PDF].
  12. K. Sansanaphongpricha, M. C. DeSantis, H. Chen, W. Cheng, K. Sun, B. Wen, and D. Sun. (2016). Multibuilding Block Janus Synthesized by Seed-Mediated Self-Assembly for Enhanced Photothermal Effects and Colored Brownian Motion in an Optical Trap. Small. doi: 10.1002/smll.201602569. [Abstract][PDF].
  13. X. Hou, M. C. DeSantis, C. Tian, and W. Cheng. (2016). Optical Manipulation of a Single Human Virus for Study of Viral-Cell Interactions. Proc. of SPIE 9922: 992212. doi: 10.1117/12.2239051. [Abstract][PDF].
  14. M. C. DeSantis, J. H. Kim, H. Song, Per Johan Klasse, and W. Cheng. (2016). Quantitative Correlation between Infectivity and Gp120 Density on HIV-1 Virions Revealed by Optical Trapping Virometry. J. Biol. Chem. 291: 13088-13097. doi: 10.1074/jbc.M116.729210. [Abstract][PDF].
  15. Y. Pang, H. Song, and W. Cheng. (2016). Using Optical Trap to Measure the Refractive Index of a Single Animal Virus in Culture Fluid with High Precision. Biomedical Optics Express 7: 1672-1689. [Abstract][PDF].
  16. B. Koh, and W. Cheng. (2016). The Kinetics of Single-Walled Carbon Nanotube Aggregation in Aqueous Media Is Sensitive to Surface Charge . J. Carbon Research doi: 10.3390/c2010006.[Abstract][PDF]
  17. B. Koh, and W. Cheng. (2015). The Impact of Sonication on the Surface Quality of Single-Walled Carbon Nanotubes. J. Pharm. Sci. doi: 10.1002/jps.24483.[Abstract][PDF]
  18. W. Cheng. (2015). Mechanisms of HCV NS3 Helicase Monitored by Optical Tweezers. In: Boudvillain M. RNA Remodeling Proteins. Methods in Molecular Biology Springer. 1259: 229-255.[Abstract][PDF]
  19. B. Koh, and W. Cheng. (2014). Mechanisms of Carbon Nanotube Aggregation and the Reversion of Carbon Nanotube Aggregates in Aqueous Medium. Langmuir 30: 10899-10909. [Full Text] [PDF]
  20. Y. Pang, H. Song, J. H. Kim, X. Hou, and W. Cheng. (2014). Optical Trapping of Individual Human Immunodeficiency Viruses in Culture Fluid Reveals Heterogeneity with Single-Molecule Resolution. Nature Nanotechnology 9: 624-630. [Abstract] [PDF].
  21. J. H. Kim, H. Song, J. L. Austin, and W. Cheng. (2013). Optimized Infectivity of the Cell-Free Single-Cycle Human Immunodeficiency Viruses Type 1 (HIV-1) and Its Restriction by Host Cells. PLOS One 8(6): e67170. [ Full Text ] [ PDF ]
  22. S. G. Arunajadai, and W. Cheng. (2013). Step Detection in Single-Molecule Real Time Trajectories Embedded in Correlated Noise. PLOS One 8(3): e59279. [ Full Text ] [ PDF ]
  23. B. Koh, G. Kim, H. Yoon, J. B. Park, R. Kopelman, and W. Cheng. (2012). Fluorophore and Dye-Assisted Dispersion of Carbon Nanotubes in Aqueous Solution. Langmuir 28: 11676-11686. [ Full Text ] [ PDF ]
  24. X. Hou, and W. Cheng. (2012). Detection of Single Fluorescent Proteins inside Eukaryotic Cells Using Two-photon Fluorescence. Biomed. Opt. Express 3: 340-353. [ Abstract ] [ PDF ]
  25. W. Cheng, S. G. Arunajadai, J. R. Moffitt, I. Tinoco Jr., and C. Bustamante. (2011). Single Base Pair Unwinding and Asynchronous RNA Release by the HCV NS3 Helicase. Science 333: 1746-1749. [ Abstract ] [ Full Text ] [ PDF ]
  26. X. Hou, and W. Cheng. (2011). Single-Molecule Detection Using Continuous-Wave Excitation of Two-Photon Fluorescence. Opt. Lett. 36: 3185-3187. (Also selected for publication in the Virtual Journal for Biomedical Optics) [ Abstract ] [ PDF ]
  27. B. Koh, J. B. Park, X. Hou, and W. Cheng. (2011). Comparative Dispersion Studies of Single-Walled Carbon Nanotubes in Aqueous Solution. J. Phys. Chem. 115: 2627-2633. [Full Text] [PDF]
  28. J. Yu, W. Cheng, C. Bustamante and G. Oster. (2010). Coupling Translocation with Nucleic Acid Unwinding by NS3 Helicase. J. Mol. Biol. 404: 439-455. [Abstract] [PDF]
  29. W. Cheng, X. Hou, and F. Ye. (2010). Use Tapered Amplifier Diode Laser for Biological-Friendly High-Resolution Optical Trapping. Opt. Lett. 35: 2988-2990. (Also selected for publication in the Virtual Journal for Biomedical Optics) [ Abstract ] [ PDF ]

Before joining UM

  1. J. Vieregg, W. Cheng, C. Bustamante, and I. Tinoco, Jr. (2007). Measurement of the Effect of Monovalent Cations on RNA Hairpin Stability. J. Am. Chem. Soc. 129: 14966-14973.
  2. W. Cheng*, S. Dumont*, I. Tinoco Jr. and C. Bustamante. (2007). NS3 Helicase Actively Separates RNA Strands and Senses Sequence Barriers Ahead of the Opening Fork. Proc. Natl. Acad. Sci., USA. 104: 13954-13959. (Direct submission) *equal authorship
    • Highlighted in:
      In This Issue, Proc. Natl. Acad. Sci., USA. (2007) 104: 13853.
      Editors' Choice, Science (2007) 317: 1295.
  3. S. Dumont*, W. Cheng*, V. Serebrov, R. K. Beran, I. Tinoco Jr., A. M. Pyle, and C. Bustamante. (2006). Direct Observation of Substeps Reveals the RNA Translocation and Unwinding Mechanism of HCV NS3 Helicase and its Coordination by ATP. Nature 439: 105-108. *equal authorship
    • Highlighted in:
      News and Views, Nat. Struct. & Mol. Biol. (2006) 13: 101.
      Hepatology elsewhere, Hepatology. (2006) 43: 1392-1395.
  4. K. M. Brendza*, W. Cheng*, C. J. Fischer, M. A. Chesnik, A. Niedziela-Majka, and T. M. Lohman. (2005). Auto-inhibition of E. coli Rep Monomer Helicase Activity by its 2B-Subdomain. Proc. Natl. Acad. Sci., USA. 102: 10076-10081. (Direct submission) *equal authorship
  5. I. Rasnik, S. Myong, W. Cheng, T. M. Lohman and T. Ha. (2004). Site-specific Fluorescent Labeling of the E. coli Rep Helicase for Single-molecule Studies of DNA Binding. J. Mol. Biol. 336: 395-408.
  6. M. C. Murphy, I. Rasnik, W. Cheng, T. M. Lohman and T. Ha. (2004). A Simple Model for Single Stranded DNA Conformational Flexibility. Biophys J. 86: 2530-2537.
  7. W. Cheng, K. M. Brendza, G. H. Gauss, S. Korolev, G. Waksman and T. M. Lohman. (2002). The 2B Domain of E. coli DNA Helicase Rep is not Required for Duplex DNA Unwinding Activity. Proc. Natl. Acad. Sci., USA. 99: 16006-16011. (Direct submission)
  8. T. Ha, I. Rasnik, W. Cheng, H. P. Babcock, G. H. Gauss, T. M. Lohman and S. Chu. (2002). Initiation and Re-initiation of DNA Unwinding by the E. coli Rep Helicase. Nature 419: 638-641.
  9. W. Cheng, J. Hsieh, K. M. Brendza and T. M. Lohman. (2001). E. coli Rep Oligomers are Required to Initiate DNA Unwinding in vitro. J. Mol. Biol. 310: 327-350.
  10. W. Cheng, C. Wang, W. Chen, Y. Xu and Y. Shi. (1998). Investigating the Dielectric Effects of Channel Pore Water on the Electrostatic Barriers of the Permeation Ion by the Finite Difference Poisson-Boltzmann Method. Eur. Biophys. J. 27: 105-112. (Undergraduate publication)

Book Chapters

  • T. M. Lohman, J. Hsieh, N. K. Maluf, W. Cheng, A. L. Lucius, C. J. Fischer, K. M. Brendza, S. Korolev & G. Waksman. (2004). DNA Helicases, Motors that Move Along Nucleic Acids: Lessons from the SF1 Helicase Superfamily. In THE ENZYMES, 3rd Edition, edited by D. D. Hackney & F. Tamanoi, Volume XXIII, Energy Coupling and Molecular Motors, 304-364.