Fierke Research Group

















1: Wang D, Fierke CA. The BaeSR regulon is involved in defense against zinc toxicity in E. coli. Metallomics. 2013 Apr 27;5(4):372-83. doi: 10.1039/c3mt20217h. PubMed PMID: 23446818.

2: Wolfson NA, Pitcairn CA, Fierke CA. HDAC8 substrates: Histones and beyond. Biopolymers. 2013 Feb;99(2):112-26. doi: 10.1002/bip.22135. PubMed PMID: 23175386; PubMed Central PMCID: PMC3507420.

3: Zeng HH, Matveeva EG, Stoddard AK, Fierke CA, Thompson RB. Long Wavelength Fluorescence Ratiometric Zinc Biosensor. J Fluoresc. 2013 Jan 24. [Epub ahead of print] PubMed PMID: 23345045.

4: Zverina EA, Lamphear CL, Wright EN, Fierke CA. Recent advances in protein prenyltransferases: substrate identification, regulation, and disease interventions. Curr Opin Chem Biol. 2012 Dec;16(5-6):544-52. doi: 10.1016/j.cbpa.2012.10.015. Epub 2012 Nov 8. PubMed PMID: 23141597; PubMed Central PMCID: PMC3518607.

5: Hougland JL, Gangopadhyay SA, Fierke CA. Expansion of protein farnesyltransferase specificity using "tunable" active site interactions: development of bioengineered prenylation pathways. J Biol Chem. 2012 Nov 2;287(45):38090-100. doi: 10.1074/jbc.M112.404954. Epub 2012 Sep 19. PubMed PMID: 22992747; PubMed Central PMCID: PMC3488079.

6: Subramanian T, Pais JE, Liu S, Troutman JM, Suzuki Y, Leela Subramanian K, Fierke CA, Andres DA, Spielmann HP. Farnesyl diphosphate analogues with aryl moieties are efficient alternate substrates for protein farnesyltransferase. Biochemistry. 2012 Oct 16;51(41):8307-19. doi: 10.1021/bi3011362. Epub 2012 Oct 2. PubMed PMID: 22989235; PubMed Central PMCID: PMC3562418.

7: Howard MJ, Lim WH, Fierke CA, Koutmos M. Mitochondrial ribonuclease P structure provides insight into the evolution of catalytic strategies for precursor-tRNA 5' processing. Proc Natl Acad Sci U S A. 2012 Oct
2;109(40):16149-54. doi: 10.1073/pnas.1209062109. Epub 2012 Sep 18. PubMed PMID: 22991464; PubMed Central PMCID: PMC3479547.

8: Wang D, Hosteen O, Fierke CA. ZntR-mediated transcription of zntA responds to nanomolar intracellular free zinc. J Inorg Biochem. 2012 Jun;111:173-81. doi: 10.1016/j.jinorgbio.2012.02.008. Epub 2012 Feb 22. PubMed PMID: 22459916; PubMed Central PMCID: PMC3408962.

9: McCranor BJ, Bozym RA, Vitolo MI, Fierke CA, Bambrick L, Polster BM, Fiskum G, Thompson RB. Quantitative imaging of mitochondrial and cytosolic free zinc levels in an in vitro model of ischemia/reperfusion. J Bioenerg Biomembr. 2012
Apr;44(2):253-63. doi: 10.1007/s10863-012-9427-2. Epub 2012 Mar 20. PubMed PMID:
22430627; PubMed Central PMCID: PMC3539817.

10: Cheriyan M, Toone EJ, Fierke CA. Improving upon nature: active site
remodeling produces highly efficient aldolase activity toward hydrophobic
electrophilic substrates. Biochemistry. 2012 Feb 28;51(8):1658-68. doi:
10.1021/bi201899b. Epub 2012 Feb 16. PubMed PMID: 22316217; PubMed Central PMCID:

11: Wang Y, Liu X, Schneider B, Zverina EA, Russ K, Wijeyesakere SJ, Fierke CA,
Richardson RJ, Philbert MA. Mixed inhibition of adenosine deaminase activity by
1,3-dinitrobenzene: a model for understanding cell-selective neurotoxicity in
chemically-induced energy deprivation syndromes in brain. Toxicol Sci. 2012
Feb;125(2):509-21. doi: 10.1093/toxsci/kfr317. Epub 2011 Nov 21. PubMed PMID:
22106038; PubMed Central PMCID: PMC3262860.

12: Yang Y, Wang B, Ucisik MN, Cui G, Fierke CA, Merz KM Jr. Insights into the
mechanistic dichotomy of the protein farnesyltransferase peptide substrates CVIM
and CVLS. J Am Chem Soc. 2012 Jan 18;134(2):820-3. doi: 10.1021/ja209650h. Epub
2012 Jan 6. PubMed PMID: 22206225; PubMed Central PMCID: PMC3277741.

13: Cheriyan M, Walters MJ, Kang BD, Anzaldi LL, Toone EJ, Fierke CA. Directed
evolution of a pyruvate aldolase to recognize a long chain acyl substrate. Bioorg
Med Chem. 2011 Nov 1;19(21):6447-53. doi: 10.1016/j.bmc.2011.08.056. Epub 2011
Aug 30. PubMed PMID: 21944547; PubMed Central PMCID: PMC3209416.

14: London N, Lamphear CL, Hougland JL, Fierke CA, Schueler-Furman O.
Identification of a novel class of farnesylation targets by structure-based
modeling of binding specificity. PLoS Comput Biol. 2011 Oct;7(10):e1002170. doi:
10.1371/journal.pcbi.1002170. Epub 2011 Oct 6. PubMed PMID: 21998565; PubMed
Central PMCID: PMC3188499.

15: Wang D, Hurst TK, Thompson RB, Fierke CA. Genetically encoded ratiometric
biosensors to measure intracellular exchangeable zinc in Escherichia coli. J
Biomed Opt. 2011 Aug;16(8):087011. doi: 10.1117/1.3613926. PubMed PMID: 21895338;
PubMed Central PMCID: PMC3166341.

16: Marvin MC, Walker SC, Fierke CA, Engelke DR. Binding and cleavage of
unstructured RNA by nuclear RNase P. RNA. 2011 Aug;17(8):1429-40. doi:
10.1261/rna.2633611. Epub 2011 Jun 10. PubMed PMID: 21665997; PubMed Central
PMCID: PMC3153968.

17: Koutmou KS, Day-Storms JJ, Fierke CA. The RNR motif of B. subtilis RNase P
protein interacts with both PRNA and pre-tRNA to stabilize an active conformer.
RNA. 2011 Jul;17(7):1225-35. doi: 10.1261/rna.2742511. Epub 2011 May 27. PubMed
PMID: 21622899; PubMed Central PMCID: PMC3138560.

18: Wands AM, Wang N, Lum JK, Hsieh J, Fierke CA, Mapp AK. Transient-state
kinetic analysis of transcriptional activator·DNA complexes interacting with a
key coactivator. J Biol Chem. 2011 May 6;286(18):16238-45. doi:
10.1074/jbc.M110.207589. Epub 2011 Feb 12. PubMed PMID: 21317429; PubMed Central
PMCID: PMC3091231.

19: Haider S, Joseph CG, Neidle S, Fierke CA, Fuchter MJ. On the function of the
internal cavity of histone deacetylase protein 8: R37 is a crucial residue for
catalysis. Bioorg Med Chem Lett. 2011 Apr 1;21(7):2129-32. doi:
10.1016/j.bmcl.2011.01.128. Epub 2011 Feb 2. PubMed PMID: 21320778; PubMed
Central PMCID: PMC3286521.

20: Cole KE, Gattis SG, Angell HD, Fierke CA, Christianson DW. Structure of the
metal-dependent deacetylase LpxC from Yersinia enterocolitica complexed with the
potent inhibitor CHIR-090 . Biochemistry. 2011 Jan 18;50(2):258-65. Epub 2010 Dec
20. PubMed PMID: 21171638; PubMed Central PMCID: PMC3070812.

21: Gattis SG, Hernick M, Fierke CA. Active site metal ion in
UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase (LpxC) switches
between Fe(II) and Zn(II) depending on cellular conditions. J Biol Chem. 2010 Oct
29;285(44):33788-96. doi: 10.1074/jbc.M110.147173. Epub 2010 Aug 13. PubMed PMID:
20709752; PubMed Central PMCID: PMC2962478.

22: Hsieh J, Koutmou KS, Rueda D, Koutmos M, Walter NG, Fierke CA. A divalent
cation stabilizes the active conformation of the B. subtilis RNase P x pre-tRNA
complex: a role for an inner-sphere metal ion in RNase P. J Mol Biol. 2010 Jul
2;400(1):38-51. doi: 10.1016/j.jmb.2010.04.050. Epub 2010 Apr 29. PubMed PMID:
20434461; PubMed Central PMCID: PMC2939038.

23: Dowling DP, Gattis SG, Fierke CA, Christianson DW. Structures of
metal-substituted human histone deacetylase 8 provide mechanistic inferences on
biological function . Biochemistry. 2010 Jun 22;49(24):5048-56. doi:
10.1021/bi1005046. PubMed PMID: 20545365; PubMed Central PMCID: PMC2895166.

24: Hernick M, Gattis SG, Penner-Hahn JE, Fierke CA. Activation of Escherichia
coli UDP-3-O-[(R)-3-hydroxymyristoyl]-N-acetylglucosamine deacetylase by Fe2+
yields a more efficient enzyme with altered ligand affinity. Biochemistry. 2010
Mar 16;49(10):2246-55. doi: 10.1021/bi902066t. PubMed PMID: 20136146; PubMed
Central PMCID: PMC2884013.

25: Gantt SL, Joseph CG, Fierke CA. Activation and inhibition of histone
deacetylase 8 by monovalent cations. J Biol Chem. 2010 Feb 26;285(9):6036-43.
doi: 10.1074/jbc.M109.033399. Epub 2009 Dec 22. PubMed PMID: 20029090; PubMed
Central PMCID: PMC2825397.

26: Koutmou KS, Zahler NH, Kurz JC, Campbell FE, Harris ME, Fierke CA.
Protein-precursor tRNA contact leads to sequence-specific recognition of 5'
leaders by bacterial ribonuclease P. J Mol Biol. 2010 Feb 12;396(1):195-208. doi:
10.1016/j.jmb.2009.11.039. Epub 2009 Nov 28. PubMed PMID: 19932118; PubMed
Central PMCID: PMC2829246.

27: Koutmou KS, Casiano-Negroni A, Getz MM, Pazicni S, Andrews AJ, Penner-Hahn
JE, Al-Hashimi HM, Fierke CA. NMR and XAS reveal an inner-sphere metal binding
site in the P4 helix of the metallo-ribozyme ribonuclease P. Proc Natl Acad Sci U
S A. 2010 Feb 9;107(6):2479-84. doi: 10.1073/pnas.0906319107. Epub 2010 Jan 25.
PubMed PMID: 20133747; PubMed Central PMCID: PMC2823894.

28: Hurst TK, Wang D, Thompson RB, Fierke CA. Carbonic anhydrase II-based metal
ion sensing: Advances and new perspectives. Biochim Biophys Acta. 2010
Feb;1804(2):393-403. doi: 10.1016/j.bbapap.2009.09.031. Epub 2009 Oct 8. Review.
PubMed PMID: 19818877; PubMed Central PMCID: PMC2818387.

29: Krzysiak AJ, Aditya AV, Hougland JL, Fierke CA, Gibbs RA. Synthesis and
screening of a CaaL peptide library versus FTase reveals a surprising number of
substrates. Bioorg Med Chem Lett. 2010 Jan 15;20(2):767-70. doi:
10.1016/j.bmcl.2009.11.011. Epub 2009 Nov 12. PubMed PMID: 20005705; PubMed
Central PMCID: PMC2922960.

30: Hougland JL, Hicks KA, Hartman HL, Kelly RA, Watt TJ, Fierke CA.
Identification of novel peptide substrates for protein farnesyltransferase
reveals two substrate classes with distinct sequence selectivities. J Mol Biol.
2010 Jan 8;395(1):176-90. doi: 10.1016/j.jmb.2009.10.038. Epub 2009 Oct 28.
PubMed PMID: 19878682; PubMed Central PMCID: PMC2916699.

31: Johnson KM, Swenson L, Opipari AW Jr, Reuter R, Zarrabi N, Fierke CA, Börsch
M, Glick GD. Mechanistic basis for differential inhibition of the F1Fo-ATPase by
aurovertin. Biopolymers. 2009 Oct;91(10):830-40. doi: 10.1002/bip.21262. PubMed
PMID: 19462418; PubMed Central PMCID: PMC2757082.

32: Hsieh J, Fierke CA. Conformational change in the Bacillus subtilis RNase P
holoenzyme--pre-tRNA complex enhances substrate affinity and limits cleavage
rate. RNA. 2009 Aug;15(8):1565-77. doi: 10.1261/rna.1639409. Epub 2009 Jun 23.
PubMed PMID: 19549719; PubMed Central PMCID: PMC2714742.

33: Hougland JL, Fierke CA. Getting a handle on protein prenylation. Nat Chem
Biol. 2009 Apr;5(4):197-8. doi: 10.1038/nchembio0409-197. PubMed PMID: 19295521.

34: Hougland JL, Lamphear CL, Scott SA, Gibbs RA, Fierke CA. Context-dependent
substrate recognition by protein farnesyltransferase. Biochemistry. 2009 Mar
3;48(8):1691-701. doi: 10.1021/bi801710g. PubMed PMID: 19199818; PubMed Central
PMCID: PMC2765569.

35: Hsieh J, Walker SC, Fierke CA, Engelke DR. Pre-tRNA turnover catalyzed by the
yeast nuclear RNase P holoenzyme is limited by product release. RNA. 2009
Feb;15(2):224-34. doi: 10.1261/rna.1309409. Epub 2008 Dec 17. PubMed PMID:
19095620; PubMed Central PMCID: PMC2648709.

36: Dowling DP, Gantt SL, Gattis SG, Fierke CA, Christianson DW. Structural
studies of human histone deacetylase 8 and its site-specific variants complexed
with substrate and inhibitors. Biochemistry. 2008 Dec 23;47(51):13554-63. doi:
10.1021/bi801610c. PubMed PMID: 19053282; PubMed Central PMCID: PMC2635894.

37: Lipton AS, Heck RW, Hernick M, Fierke CA, Ellis PD. Residue ionization in
LpxC directly observed by 67Zn NMR spectroscopy. J Am Chem Soc. 2008 Sep
24;130(38):12671-9. doi: 10.1021/ja801776c. Epub 2008 Aug 30. PubMed PMID:
18761443; PubMed Central PMCID: PMC2652226.

38: Mason AB, Judson GL, Bravo MC, Edelstein A, Byrne SL, James NG, Roush ED,
Fierke CA, Bobst CE, Kaltashov IA, Daughtery MA. Evolution reversed: the ability
to bind iron restored to the N-lobe of the murine inhibitor of carbonic anhydrase
by strategic mutagenesis. Biochemistry. 2008 Sep 16;47(37):9847-55. doi:
10.1021/bi801133d. Epub 2008 Aug 20. PubMed PMID: 18712936; PubMed Central PMCID:

39: Walters MJ, Srikannathasan V, McEwan AR, Naismith JH, Fierke CA, Toone EJ.
Characterization and crystal structure of Escherichia coli KDPGal aldolase.
Bioorg Med Chem. 2008 Jan 15;16(2):710-20. Epub 2007 Oct 18. PubMed PMID:
17981470; PubMed Central PMCID: PMC3326530.

40: Bozym R, Hurst TK, Westerberg N, Stoddard A, Fierke CA, Frederickson CJ,
Thompson RB. Determination of zinc using carbonic anhydrase-based fluorescence
biosensors. Methods Enzymol. 2008;450:287-309. doi:
10.1016/S0076-6879(08)03414-9. PubMed PMID: 19152866.

41: Hernick M, Fierke CA. A method to assay inhibitors of lipopolysaccharide
synthesis. Methods Mol Med. 2008;142:143-54. doi: 10.1007/978-1-59745-246-5_12.
PubMed PMID: 18437312.

42: Smith JK, Hsieh J, Fierke CA. Importance of RNA-protein interactions in
bacterial ribonuclease P structure and catalysis. Biopolymers. 2007 Dec
5-15;87(5-6):329-38. Review. PubMed PMID: 17868095.

43: Inamdar MV, Lastoskie CM, Fierke CA, Sastry AM. Mobile trap algorithm for
zinc detection using protein sensors. J Chem Phys. 2007 Nov 14;127(18):185102.
PubMed PMID: 18020665.

44: Cheriyan M, Toone EJ, Fierke CA. Mutagenesis of the phosphate-binding pocket
of KDPG aldolase enhances selectivity for hydrophobic substrates. Protein Sci.
2007 Nov;16(11):2368-77. PubMed PMID: 17962400; PubMed Central PMCID: PMC2211689.

45: Krzysiak AJ, Scott SA, Hicks KA, Fierke CA, Gibbs RA. Evaluation of protein
farnesyltransferase substrate specificity using synthetic peptide libraries.
Bioorg Med Chem Lett. 2007 Oct 15;17(20):5548-51. Epub 2007 Aug 16. PubMed PMID:
17804232; PubMed Central PMCID: PMC2077820.

46: Krzysiak AJ, Rawat DS, Scott SA, Pais JE, Handley M, Harrison ML, Fierke CA,
Gibbs RA. Combinatorial modulation of protein prenylation. ACS Chem Biol. 2007
Jun 15;2(6):385-9. Epub 2007 May 25. PubMed PMID: 17530735; PubMed Central PMCID:

47: Niranjanakumari S, Day-Storms JJ, Ahmed M, Hsieh J, Zahler NH, Venters RA,
Fierke CA. Probing the architecture of the B. subtilis RNase P holoenzyme active
site by cross-linking and affinity cleavage. RNA. 2007 Apr;13(4):521-35. Epub
2007 Feb 13. PubMed PMID: 17299131; PubMed Central PMCID: PMC1831860.

48: Getz MM, Andrews AJ, Fierke CA, Al-Hashimi HM. Structural plasticity and Mg2+
binding properties of RNase P P4 from combined analysis of NMR residual dipolar
couplings and motionally decoupled spin relaxation. RNA. 2007 Feb;13(2):251-66.
Epub 2006 Dec 28. PubMed PMID: 17194721; PubMed Central PMCID: PMC1781369.

49: Hernick M, Fierke CA. Catalytic mechanism and molecular recognition of E.
coli UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase probed by
mutagenesis. Biochemistry. 2006 Dec 26;45(51):15240-8. PubMed PMID: 17176046.

50: Hernick M, Fierke CA. Molecular recognition by Escherichia coli
UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase is modulated by
bound metal ions. Biochemistry. 2006 Dec 12;45(49):14573-81. PubMed PMID:

51: Pais JE, Bowers KE, Fierke CA. Measurement of the alpha-secondary kinetic
isotope effect for the reaction catalyzed by mammalian protein
farnesyltransferase. J Am Chem Soc. 2006 Nov 29;128(47):15086-7. PubMed PMID:

52: Gennadios HA, Whittington DA, Li X, Fierke CA, Christianson DW. Mechanistic
inferences from the binding of ligands to LpxC, a metal-dependent deacetylase.
Biochemistry. 2006 Jul 4;45(26):7940-8. PubMed PMID: 16800620; PubMed Central
PMCID: PMC2505344.

53: Johnson KM, Cleary J, Fierke CA, Opipari AW Jr, Glick GD. Mechanistic basis
for therapeutic targeting of the mitochondrial F1F0-ATPase. ACS Chem Biol. 2006
Jun 20;1(5):304-8. PubMed PMID: 17163759.

54: Xiao S, Hsieh J, Nugent RL, Coughlin DJ, Fierke CA, Engelke DR. Functional
characterization of the conserved amino acids in Pop1p, the largest common
protein subunit of yeast RNases P and MRP. RNA. 2006 Jun;12(6):1023-37. Epub 2006
Apr 17. PubMed PMID: 16618965; PubMed Central PMCID: PMC1464857.

55: Gantt SL, Gattis SG, Fierke CA. Catalytic activity and inhibition of human
histone deacetylase 8 is dependent on the identity of the active site metal ion.
Biochemistry. 2006 May 16;45(19):6170-8. PubMed PMID: 16681389.

56: Fullerton SW, Griffiths JS, Merkel AB, Cheriyan M, Wymer NJ, Hutchins MJ,
Fierke CA, Toone EJ, Naismith JH. Mechanism of the Class I KDPG aldolase. Bioorg
Med Chem. 2006 May 1;14(9):3002-10. Epub 2006 Jan 5. PubMed PMID: 16403639;
PubMed Central PMCID: PMC3315828.

57: Frederickson CJ, Giblin LJ, Krezel A, McAdoo DJ, Mueller RN, Zeng Y, Balaji
RV, Masalha R, Thompson RB, Fierke CA, Sarvey JM, de Valdenebro M, Prough DS,
Zornow MH. Concentrations of extracellular free zinc (pZn)e in the central
nervous system during simple anesthetization, ischemia and reperfusion. Exp
Neurol. 2006 Apr;198(2):285-93. Epub 2006 Jan 26. Erratum in: Exp Neurol. 2006
Dec;202(2):523. Muelle, R N [corrected to Mueller, R N]. PubMed PMID: 16443223.

58: Bozym RA, Thompson RB, Stoddard AK, Fierke CA. Measuring picomolar
intracellular exchangeable zinc in PC-12 cells using a ratiometric fluorescence
biosensor. ACS Chem Biol. 2006 Mar 17;1(2):103-11. PubMed PMID: 17163650.

59: Sumner JP, Westerberg NM, Stoddard AK, Hurst TK, Cramer M, Thompson RB,
Fierke CA, Kopelman R. DsRed as a highly sensitive, selective, and reversible
fluorescence-based biosensor for both Cu(+) and Cu(2+) ions. Biosens Bioelectron.
2006 Jan 15;21(7):1302-8. Epub 2005 Jul 18. PubMed PMID: 16026976.

60: Rueda D, Hsieh J, Day-Storms JJ, Fierke CA, Walter NG. The 5' leader of
precursor tRNAAsp bound to the Bacillus subtilis RNase P holoenzyme has an
extended conformation. Biochemistry. 2005 Dec 13;44(49):16130-9. PubMed PMID:

61: Hicks KA, Hartman HL, Fierke CA. Upstream polybasic region in peptides
enhances dual specificity for prenylation by both farnesyltransferase and
geranylgeranyltransferase type I. Biochemistry. 2005 Nov 22;44(46):15325-33.
PubMed PMID: 16285736.

62: Hartman HL, Hicks KA, Fierke CA. Peptide specificity of protein
prenyltransferases is determined mainly by reactivity rather than binding
affinity. Biochemistry. 2005 Nov 22;44(46):15314-24. PubMed PMID: 16285735.

63: Pais JE, Bowers KE, Stoddard AK, Fierke CA. A continuous fluorescent assay
for protein prenyltransferases measuring diphosphate release. Anal Biochem. 2005
Oct 15;345(2):302-11. PubMed PMID: 16143290.

64: Reigard SA, Zahn TJ, Haworth KB, Hicks KA, Fierke CA, Gibbs RA. Interplay of
isoprenoid and peptide substrate specificity in protein farnesyltransferase.
Biochemistry. 2005 Aug 23;44(33):11214-23. PubMed PMID: 16101305.

65: Xiao S, Day-Storms JJ, Srisawat C, Fierke CA, Engelke DR. Characterization of
conserved sequence elements in eukaryotic RNase P RNA reveals roles in holoenzyme
assembly and tRNA processing. RNA. 2005 Jun;11(6):885-96. Epub 2005 May 4. PubMed
PMID: 15872187; PubMed Central PMCID: PMC1370773.

66: Hernick M, Gennadios HA, Whittington DA, Rusche KM, Christianson DW, Fierke
CA. UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase functions
through a general acid-base catalyst pair mechanism. J Biol Chem. 2005 Apr
29;280(17):16969-78. Epub 2005 Feb 10. PubMed PMID: 15705580.

67: Hernick M, Fierke CA. Zinc hydrolases: the mechanisms of zinc-dependent
deacetylases. Arch Biochem Biophys. 2005 Jan 1;433(1):71-84. Review. PubMed PMID:

68: Day-Storms JJ, Niranjanakumari S, Fierke CA. Ionic interactions between PRNA
and P protein in Bacillus subtilis RNase P characterized using a
magnetocapture-based assay. RNA. 2004 Oct;10(10):1595-608. Epub 2004 Aug 30.
PubMed PMID: 15337847; PubMed Central PMCID: PMC1370646.

69: Griffiths JS, Cheriyan M, Corbell JB, Pocivavsek L, Fierke CA, Toone EJ. A
bacterial selection for the directed evolution of pyruvate aldolases. Bioorg Med
Chem. 2004 Aug 1;12(15):4067-74. PubMed PMID: 15246084.

70: Hartman HL, Bowers KE, Fierke CA. Lysine beta311 of protein
geranylgeranyltransferase type I partially replaces magnesium. J Biol Chem. 2004
Jul 16;279(29):30546-53. Epub 2004 May 5. PubMed PMID: 15131129.

71: Bowers KE, Fierke CA. Positively charged side chains in protein
farnesyltransferase enhance catalysis by stabilizing the formation of the
diphosphate leaving group. Biochemistry. 2004 May 11;43(18):5256-65. PubMed PMID:

72: McCall KA, Fierke CA. Probing determinants of the metal ion selectivity in
carbonic anhydrase using mutagenesis. Biochemistry. 2004 Apr 6;43(13):3979-86.
PubMed PMID: 15049705.

73: Hsieh J, Andrews AJ, Fierke CA. Roles of protein subunits in RNA-protein
complexes: lessons from ribonuclease P. Biopolymers. 2004 Jan;73(1):79-89.
Review. PubMed PMID: 14691942.

74: Pickett JS, Bowers KE, Fierke CA. Mutagenesis studies of protein
farnesyltransferase implicate aspartate beta 352 as a magnesium ligand. J Biol
Chem. 2003 Dec 19;278(51):51243-50. Epub 2003 Oct 7. PubMed PMID: 14532266.

75: Zeng HH, Thompson RB, Maliwal BP, Fones GR, Moffett JW, Fierke CA. Real-time
determination of picomolar free Cu(II) in seawater using a fluorescence-based
fiber optic biosensor. Anal Chem. 2003 Dec 15;75(24):6807-12. PubMed PMID:

76: Tobin DA, Pickett JS, Hartman HL, Fierke CA, Penner-Hahn JE. Structural
characterization of the zinc site in protein farnesyltransferase. J Am Chem Soc.
2003 Aug 20;125(33):9962-9. PubMed PMID: 12914459.

77: Pickett JS, Bowers KE, Hartman HL, Fu HW, Embry AC, Casey PJ, Fierke CA.
Kinetic studies of protein farnesyltransferase mutants establish active substrate
conformation. Biochemistry. 2003 Aug 19;42(32):9741-8. PubMed PMID: 12911316.

78: Whittington DA, Rusche KM, Shin H, Fierke CA, Christianson DW. Crystal
structure of LpxC, a zinc-dependent deacetylase essential for endotoxin
biosynthesis. Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8146-50. Epub 2003 Jun
20. PubMed PMID: 12819349; PubMed Central PMCID: PMC166197.

79: Shao F, Vacratsis PO, Bao Z, Bowers KE, Fierke CA, Dixon JE. Biochemical
characterization of the Yersinia YopT protease: cleavage site and recognition
elements in Rho GTPases. Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):904-9. Epub
2003 Jan 21. PubMed PMID: 12538863; PubMed Central PMCID: PMC298699.

80: McClure CP, Rusche KM, Peariso K, Jackman JE, Fierke CA, Penner-Hahn JE.
EXAFS studies of the zinc sites of UDP-(3-O-acyl)-N-acetylglucosamine deacetylase
(LpxC). J Inorg Biochem. 2003 Feb 1;94(1-2):78-85. PubMed PMID: 12620676.

81: Pirrung MC, Tumey LN, Raetz CR, Jackman JE, Snehalatha K, McClerren AL,
Fierke CA, Gantt SL, Rusche KM. Inhibition of the antibacterial target
UDP-(3-O-acyl)-N-acetylglucosamine deacetylase (LpxC): isoxazoline zinc amidase
inhibitors bearing diverse metal binding groups. J Med Chem. 2002 Sep
12;45(19):4359-70. PubMed PMID: 12213077.

82: Ridderstråle Y, Fierke CA, Roush ED, Wistrand PJ. Localization of a protein
inhibitor of carbonic anhydrase in pig tissues. Acta Physiol Scand. 2002
Sep;176(1):27-31. PubMed PMID: 12193216.

83: Kurz JC, Fierke CA. The affinity of magnesium binding sites in the Bacillus
subtilis RNase P x pre-tRNA complex is enhanced by the protein subunit.
Biochemistry. 2002 Jul 30;41(30):9545-58. PubMed PMID: 12135377.

84: Chehade KA, Kiegiel K, Isaacs RJ, Pickett JS, Bowers KE, Fierke CA, Andres
DA, Spielmann HP. Photoaffinity analogues of farnesyl pyrophosphate transferable
by protein farnesyl transferase. J Am Chem Soc. 2002 Jul 17;124(28):8206-19.
PubMed PMID: 12105898.

85: Crary SM, Kurz JC, Fierke CA. Specific phosphorothioate substitutions probe
the active site of Bacillus subtilis ribonuclease P. RNA. 2002 Jul;8(7):933-47.
PubMed PMID: 12166648; PubMed Central PMCID: PMC1370310.

86: Griffiths JS, Wymer NJ, Njolito E, Niranjanakumari S, Fierke CA, Toone EJ.
Cloning, isolation and characterization of the Thermotoga maritima KDPG aldolase.
Bioorg Med Chem. 2002 Mar;10(3):545-50. PubMed PMID: 11814840.

87: Madder RD, Kim CY, Chandra PP, Doyon JB, Baird TA Jr, Fierke CA, Christianson
DW, Voet JG, Jain A. Twisted amides inferred from QSAR analysis of hydrophobicity
and electronic effects on the affinity of fluoroaromatic inhibitors of carbonic
anhydrase. J Org Chem. 2002 Jan 25;67(2):582-4. PubMed PMID: 11798333.

88: Xiao S, Scott F, Fierke CA, Engelke DR. Eukaryotic ribonuclease P: a
plurality of ribonucleoprotein enzymes. Annu Rev Biochem. 2002;71:165-89. Epub
2001 Nov 9. Review. PubMed PMID: 12045094.

89: Fierke CA, Thompson RB. Fluorescence-based biosensing of zinc using carbonic
anhydrase. Biometals. 2001 Sep-Dec;14(3-4):205-22. Review. PubMed PMID: 11831457.

90: DiTusa CA, McCall KA, Christensen T, Mahapatro M, Fierke CA, Toone EJ.
Thermodynamics of metal ion binding. 2. Metal ion binding by carbonic anhydrase
variants. Biochemistry. 2001 May 8;40(18):5345-51. PubMed PMID: 11330997.

91: DiTusa CA, Christensen T, McCall KA, Fierke CA, Toone EJ. Thermodynamics of
metal ion binding. 1. Metal ion binding by wild-type carbonic anhydrase.
Biochemistry. 2001 May 8;40(18):5338-44. PubMed PMID: 11330996.

92: Henkels CH, Kurz JC, Fierke CA, Oas TG. Linked folding and anion binding of
the Bacillus subtilis ribonuclease P protein. Biochemistry. 2001 Mar
6;40(9):2777-89. PubMed PMID: 11258888.

93: Fang XW, Yang XJ, Littrell K, Niranjanakumari S, Thiyagarajan P, Fierke CA,
Sosnick TR, Pan T. The Bacillus subtilis RNase P holoenzyme contains two RNase P
RNA and two RNase P protein subunits. RNA. 2001 Feb;7(2):233-41. PubMed PMID:
11233980; PubMed Central PMCID: PMC1370081.

94: Hightower KE, Casey PJ, Fierke CA. Farnesylation of nonpeptidic thiol
compounds by protein farnesyltransferase. Biochemistry. 2001 Jan
30;40(4):1002-10. PubMed PMID: 11170422.

95: Jackman JE, Raetz CR, Fierke CA. Site-directed mutagenesis of the bacterial
metalloamidase UDP-(3-O-acyl)-N-acetylglucosamine deacetylase (LpxC).
Identification of the zinc binding site. Biochemistry. 2001 Jan 16;40(2):514-23.
PubMed PMID: 11148046.

96: Wymer N, Buchanan LV, Henderson D, Mehta N, Botting CH, Pocivavsek L, Fierke
CA, Toone EJ, Naismith JH. Directed evolution of a new catalytic site in
2-keto-3-deoxy-6-phosphogluconate aldolase from Escherichia coli. Structure. 2001
Jan 10;9(1):1-9. PubMed PMID: 11342129.

97: Cox JD, Hunt JA, Compher KM, Fierke CA, Christianson DW. Structural influence
of hydrophobic core residues on metal binding and specificity in carbonic
anhydrase II. Biochemistry. 2000 Nov 14;39(45):13687-94. PubMed PMID: 11076507.

98: Spence RA, Hightower KE, Terry KL, Beese LS, Fierke CA, Casey PJ. Conversion
of Tyr361 beta to Leu in mammalian protein farnesyltransferase impairs product
release but not substrate recognition. Biochemistry. 2000 Nov 14;39(45):13651-9.
PubMed PMID: 11076503.

99: Saderholm MJ, Hightower KE, Fierke CA. Role of metals in the reaction
catalyzed by protein farnesyltransferase. Biochemistry. 2000 Oct
10;39(40):12398-405. PubMed PMID: 11015220.

100: Kurz JC, Fierke CA. Ribonuclease P: a ribonucleoprotein enzyme. Curr Opin
Chem Biol. 2000 Oct;4(5):553-8. Review. PubMed PMID: 11006544.

101: McCall KA, Fierke CA. Colorimetric and fluorimetric assays to quantitate
micromolar concentrations of transition metals. Anal Biochem. 2000 Sep
10;284(2):307-15. PubMed PMID: 10964414.

102: Ziehler WA, Day JJ, Fierke CA, Engelke DR. Effects of 5' leader and 3'
trailer structures on pre-tRNA processing by nuclear RNase P. Biochemistry. 2000
Aug 15;39(32):9909-16. PubMed PMID: 10933810.

103: Doyon JB, Hansen EA, Kim CY, Chang JS, Christianson DW, Madder RD, Voet JG,
Baird TA Jr, Fierke CA, Jain A. Linear free energy relationships implicate three
modes of binding for fluoroaromatic inhibitors to a mutant of carbonic anhydrase
II. Org Lett. 2000 Aug 10;2(16):2557-8. PubMed PMID: 10956546.

104: Doyon JB, Hansen EA, Kim CY, Chang JS, Christianson DW, Madder RD, Voet JG,
Baird TA Jr, Fierke CA, Jain A. Linear free energy relationships implicate three
modes of binding for fluoroaromatic inhibitors to a mutant of carbonic anhydrase
II. Org Lett. 2000 May 4;2(9):1189-92. Erratum in: Org Lett 2000 Aug
10;2(16):2557. PubMed PMID: 10810704.

105: McCall KA, Huang C, Fierke CA. Function and mechanism of zinc
metalloenzymes. J Nutr. 2000 May;130(5S Suppl):1437S-46S. Review. PubMed PMID:

106: Jackman JE, Fierke CA, Tumey LN, Pirrung M, Uchiyama T, Tahir SH, Hindsgaul
O, Raetz CR. Antibacterial agents that target lipid A biosynthesis in
gram-negative bacteria. Inhibition of diverse
UDP-3-O-(r-3-hydroxymyristoyl)-n-acetylglucosamine deacetylases by substrate
analogs containing zinc binding motifs. J Biol Chem. 2000 Apr 14;275(15):11002-9.
PubMed PMID: 10753902.

107: Burton RE, Hunt JA, Fierke CA, Oas TG. Novel disulfide engineering in human
carbonic anhydrase II using the PAIRWISE side-chain geometry database. Protein
Sci. 2000 Apr;9(4):776-85. PubMed PMID: 10794421; PubMed Central PMCID:

108: Huang C, Hightower KE, Fierke CA. Mechanistic studies of rat protein
farnesyltransferase indicate an associative transition state. Biochemistry. 2000
Mar 14;39(10):2593-602. PubMed PMID: 10704208.

109: Thompson RB, Whetsell WO Jr, Maliwal BP, Fierke CA, Frederickson CJ.
Fluorescence microscopy of stimulated Zn(II) release from organotypic cultures of
mammalian hippocampus using a carbonic anhydrase-based biosensor system. J
Neurosci Methods. 2000 Mar 1;96(1):35-45. PubMed PMID: 10704669.

110: Hunt JA, Lesburg CA, Christianson DW, Thompson RB, Fierke CA. Active-site
engineering of carbonic anhydrase and its application to biosensors. EXS.
2000;(90):221-40. Review. PubMed PMID: 11268518.

111: Alber BE, Colangelo CM, Dong J, Stålhandske CM, Baird TT, Tu C, Fierke CA,
Silverman DN, Scott RA, Ferry JG. Kinetic and spectroscopic characterization of
the gamma-carbonic anhydrase from the methanoarchaeon Methanosarcina thermophila.
Biochemistry. 1999 Oct 5;38(40):13119-28. PubMed PMID: 10529183.

112: Hunt JA, Ahmed M, Fierke CA. Metal binding specificity in carbonic anhydrase
is influenced by conserved hydrophobic core residues. Biochemistry. 1999 Jul
13;38(28):9054-62. PubMed PMID: 10413479.

113: Hightower KE, Fierke CA. Zinc-catalyzed sulfur alkyation:insights from
protein farnesyltransferase. Curr Opin Chem Biol. 1999 Apr;3(2):176-81. Review.
PubMed PMID: 10226042.

114: Jackman JE, Raetz CR, Fierke CA.
UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase of Escherichia
coli is a zinc metalloenzyme. Biochemistry. 1999 Feb 9;38(6):1902-11. PubMed
PMID: 10026271.

115: Thompson RB, Maliwal BP, Fierke CA. Selectivity and sensitivity of
fluorescence lifetime-based metal ion biosensing using a carbonic anhydrase
transducer. Anal Biochem. 1999 Feb 1;267(1):185-95. PubMed PMID: 9918670.

116: Ogura T, Inoue K, Tatsuta T, Suzaki T, Karata K, Young K, Su LH, Fierke CA,
Jackman JE, Raetz CR, Coleman J, Tomoyasu T, Matsuzawa H. Balanced biosynthesis
of major membrane components through regulated degradation of the committed
enzyme of lipid A biosynthesis by the AAA protease FtsH (HflB) in Escherichia
coli. Mol Microbiol. 1999 Feb;31(3):833-44. PubMed PMID: 10048027.

117: Niranjanakumari S, Stams T, Crary SM, Christianson DW, Fierke CA. Protein
component of the ribozyme ribonuclease P alters substrate recognition by directly
contacting precursor tRNA. Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15212-7.
PubMed PMID: 9860948; PubMed Central PMCID: PMC28022.

118: Zimmerman KK, Scholten JD, Huang CC, Fierke CA, Hupe DJ. High-level
expression of rat farnesyl:protein transferase in Escherichia coli as a
translationally coupled heterodimer. Protein Expr Purif. 1998 Dec;14(3):395-402.
PubMed PMID: 9882574.

119: Thompson RB, Maliwal BP, Feliccia VL, Fierke CA, McCall K. Determination of
picomolar concentrations of metal ions using fluorescence anisotropy: biosensing
with a "reagentless" enzyme transducer. Anal Chem. 1998 Nov 15;70(22):4717-23.
PubMed PMID: 9844569.

120: Hightower KE, Huang CC, Casey PJ, Fierke CA. H-Ras peptide and protein
substrates bind protein farnesyltransferase as an ionized thiolate. Biochemistry.
1998 Nov 3;37(44):15555-62. PubMed PMID: 9799520.

121: Loria A, Niranjanakumari S, Fierke CA, Pan T. Recognition of a pre-tRNA
substrate by the Bacillus subtilis RNase P holoenzyme. Biochemistry. 1998 Nov
3;37(44):15466-73. PubMed PMID: 9799509.

122: Niranjanakumari S, Kurz JC, Fierke CA. Expression, purification and
characterization of the recombinant ribonuclease P protein component from
Bacillus subtilis. Nucleic Acids Res. 1998 Jul 1;26(13):3090-6. PubMed PMID:
9628904; PubMed Central PMCID: PMC147689.

123: Crary SM, Niranjanakumari S, Fierke CA. The protein component of Bacillus
subtilis ribonuclease P increases catalytic efficiency by enhancing interactions
with the 5' leader sequence of pre-tRNAAsp. Biochemistry. 1998 Jun
30;37(26):9409-16. PubMed PMID: 9649323.

124: Thompson RB, Maliwal BP, Fierke CA. Expanded dynamic range of free zinc ion
determination by fluorescence anisotropy. Anal Chem. 1998 May 1;70(9):1749-54.
doi: 10.1021/ac971199+. PubMed PMID: 21651269.

125: Stams T, Niranjanakumari S, Fierke CA, Christianson DW. Ribonuclease P
protein structure: evolutionary origins in the translational apparatus. Science.
1998 May 1;280(5364):752-5. PubMed PMID: 9563955.

126: Kurz JC, Niranjanakumari S, Fierke CA. Protein component of Bacillus
subtilis RNase P specifically enhances the affinity for precursor-tRNAAsp.
Biochemistry. 1998 Feb 24;37(8):2393-400. PubMed PMID: 9485387.

127: Lesburg CA, Huang C, Christianson DW, Fierke CA. Histidine --> carboxamide
ligand substitutions in the zinc binding site of carbonic anhydrase II alter
metal coordination geometry but retain catalytic activity. Biochemistry. 1997 Dec
16;36(50):15780-91. PubMed PMID: 9398308.

128: Hunt JA, Fierke CA. Selection of carbonic anhydrase variants displayed on
phage. Aromatic residues in zinc binding site enhance metal affinity and
equilibration kinetics. J Biol Chem. 1997 Aug 15;272(33):20364-72. PubMed PMID:

129: Wuebbens MW, Roush ED, Decastro CM, Fierke CA. Cloning, sequencing, and
recombinant expression of the porcine inhibitor of carbonic anhydrase: a novel
member of the transferrin family. Biochemistry. 1997 Apr 8;36(14):4327-36. PubMed
PMID: 9100029.

130: Baird TT Jr, Waheed A, Okuyama T, Sly WS, Fierke CA. Catalysis and
inhibition of human carbonic anhydrase IV. Biochemistry. 1997 Mar
4;36(9):2669-78. PubMed PMID: 9054574.

131: Huang CC, Casey PJ, Fierke CA. Evidence for a catalytic role of zinc in
protein farnesyltransferase. Spectroscopy of Co2+-farnesyltransferase indicates
metal coordination of the substrate thiolate. J Biol Chem. 1997 Jan
3;272(1):20-3. PubMed PMID: 8995218.

132: Jackman JE, Merz KM Jr, Fierke CA. Disruption of the active site solvent
network in carbonic anhydrase II decreases the efficiency of proton transfer.
Biochemistry. 1996 Dec 24;35(51):16421-8. PubMed PMID: 8987973.

133: Venters RA, Farmer BT 2nd, Fierke CA, Spicer LD. Characterizing the use of
perdeuteration in NMR studies of large proteins: 13C, 15N and 1H assignments of
human carbonic anhydrase II. J Mol Biol. 1996 Dec 20;264(5):1101-16. PubMed PMID:

134: Beebe JA, Kurz JC, Fierke CA. Magnesium ions are required by Bacillus
subtilis ribonuclease P RNA for both binding and cleaving precursor tRNAAsp.
Biochemistry. 1996 Aug 13;35(32):10493-505. PubMed PMID: 8756706.

135: Huang CC, Lesburg CA, Kiefer LL, Fierke CA, Christianson DW. Reversal of the
hydrogen bond to zinc ligand histidine-119 dramatically diminishes catalysis and
enhances metal equilibration kinetics in carbonic anhydrase II. Biochemistry.
1996 Mar 19;35(11):3439-46. PubMed PMID: 8639494.

136: Thompson RB, Ge Z, Patchan MW, Fierke CA. Performance enhancement of
fluorescence energy transfer-based biosensors by site-directed mutagenesis of the
transducer. J Biomed Opt. 1996 Jan 1;1(1):131-137. PubMed PMID: 23014654.

137: Ippolito JA, Nair SK, Alexander RS, Kiefer LL, Fierke CA, Christianson DW.
Structure of His94-->Asp carbonic anhydrase II in a new crystalline form reveals
a partially occupied zinc binding site. Protein Eng. 1995 Oct;8(10):975-80.
PubMed PMID: 8771178.

138: Whitty A, Fierke CA, Jencks WP. Role of binding energy with coenzyme A in
catalysis by 3-oxoacid coenzyme A transferase. Biochemistry. 1995 Sep
19;34(37):11678-89. PubMed PMID: 7547900.

139: Venters RA, Huang CC, Farmer BT 2nd, Trolard R, Spicer LD, Fierke CA.
High-level 2H/13C/15N labeling of proteins for NMR studies. J Biomol NMR. 1995
Jun;5(4):339-44. PubMed PMID: 7647552.

140: Ippolito JA, Baird TT Jr, McGee SA, Christianson DW, Fierke CA.
Structure-assisted redesign of a protein-zinc-binding site with femtomolar
affinity. Proc Natl Acad Sci U S A. 1995 May 23;92(11):5017-21. PubMed PMID:
7761440; PubMed Central PMCID: PMC41839.

141: Nair SK, Krebs JF, Christianson DW, Fierke CA. Structural basis of inhibitor
affinity to variants of human carbonic anhydrase II. Biochemistry. 1995 Mar
28;34(12):3981-9. PubMed PMID: 7696263.

142: Fierke CA, Hammes GG. Transient kinetic approaches to enzyme mechanisms.
Methods Enzymol. 1995;249:3-37. PubMed PMID: 7791616.

143: Puttaraju M, Beebe JA, Niranjanakumari S, Been MD, Fierke CA. Generation and
characterization of circular Bacillus subtilis RNase P RNA; activation by RNase P
protein. Nucleic Acids Symp Ser. 1995;(33):92-4. PubMed PMID: 8643411.

144: Kiefer LL, Fierke CA. Functional characterization of human carbonic
anhydrase II variants with altered zinc binding sites. Biochemistry. 1994 Dec
27;33(51):15233-40. PubMed PMID: 7803385.

145: Beebe JA, Fierke CA. A kinetic mechanism for cleavage of precursor tRNA(Asp)
catalyzed by the RNA component of Bacillus subtilis ribonuclease P. Biochemistry.
1994 Aug 30;33(34):10294-304. PubMed PMID: 7520753.

146: Krebs JF, Ippolito JA, Christianson DW, Fierke CA. Structural and functional
importance of a conserved hydrogen bond network in human carbonic anhydrase II. J
Biol Chem. 1993 Dec 25;268(36):27458-66. PubMed PMID: 8262987.

147: Tweedy NB, Nair SK, Paterno SA, Fierke CA, Christianson DW. Structure and
energetics of a non-proline cis-peptidyl linkage in a proline-202-->alanine
carbonic anhydrase II variant. Biochemistry. 1993 Oct 19;32(41):10944-9. PubMed
PMID: 8218160.

148: Heming TA, Vanoye CG, Stabenau EK, Roush ED, Fierke CA, Bidani A. Inhibitor
sensitivity of pulmonary vascular carbonic anhydrase. J Appl Physiol. 1993
Oct;75(4):1642-9. PubMed PMID: 8282615.

149: Kiefer LL, Krebs JF, Paterno SA, Fierke CA. Engineering a cysteine ligand
into the zinc binding site of human carbonic anhydrase II. Biochemistry. 1993 Sep
28;32(38):9896-900. PubMed PMID: 8399158.

150: Krebs JF, Rana F, Dluhy RA, Fierke CA. Kinetic and spectroscopic studies of
hydrophilic amino acid substitutions in the hydrophobic pocket of human carbonic
anhydrase II. Biochemistry. 1993 May 4;32(17):4496-505. PubMed PMID: 8485128.

151: Alexander RS, Kiefer LL, Fierke CA, Christianson DW. Engineering the zinc
binding site of human carbonic anhydrase II: structure of the His-94-->Cys
apoenzyme in a new crystalline form. Biochemistry. 1993 Feb 16;32(6):1510-8.
PubMed PMID: 8431430.

152: Krebs JF, Fierke CA. Determinants of catalytic activity and stability of
carbonic anhydrase II as revealed by random mutagenesis. J Biol Chem. 1993 Jan
15;268(2):948-54. PubMed PMID: 8419374.

153: Roush ED, Fierke CA. Purification and characterization of a carbonic
anhydrase II inhibitor from porcine plasma. Biochemistry. 1992 Dec
15;31(49):12536-42. PubMed PMID: 1463741.

154: Adams JA, Fierke CA, Benkovic SJ. The function of amino acid residues
contacting the nicotinamide ring of NADPH in dihydrofolate reductase from
Escherichia coli. Biochemistry. 1991 Nov 19;30(46):11046-54. PubMed PMID:

155: Fierke CA, Calderone TL, Krebs JF. Functional consequences of engineering
the hydrophobic pocket of carbonic anhydrase II. Biochemistry. 1991 Nov
19;30(46):11054-63. PubMed PMID: 1657158.

156: Krebs JF, Fierke CA, Alexander RS, Christianson DW. Conformational mobility
of His-64 in the Thr-200----Ser mutant of human carbonic anhydrase II.
Biochemistry. 1991 Sep 24;30(38):9153-60. PubMed PMID: 1909891.

157: Nair SK, Calderone TL, Christianson DW, Fierke CA. Altering the mouth of a
hydrophobic pocket. Structure and kinetics of human carbonic anhydrase II mutants
at residue Val-121. J Biol Chem. 1991 Sep 15;266(26):17320-5. PubMed PMID:

158: Venters RA, Calderone TL, Spicer LD, Fierke CA. Uniform 13C isotope labeling
of proteins with sodium acetate for NMR studies: application to human carbonic
anhydrase II. Biochemistry. 1991 May 7;30(18):4491-4. PubMed PMID: 1902380.

159: Andrews J, Fierke CA, Birdsall B, Ostler G, Feeney J, Roberts GC, Benkovic
SJ. A kinetic study of wild-type and mutant dihydrofolate reductases from
Lactobacillus casei. Biochemistry. 1989 Jul 11;28(14):5743-50. PubMed PMID:

160: Fierke CA, Benkovic SJ. Probing the functional role of threonine-113 of
Escherichia coli dihydrofolate reductase for its effect on turnover efficiency,
catalysis, and binding. Biochemistry. 1989 Jan 24;28(2):478-86. PubMed PMID:

161: Benkovic SJ, Fierke CA, Naylor AM. Insights into enzyme function from
studies on mutants of dihydrofolate reductase. Science. 1988 Mar
4;239(4844):1105-10. Review. PubMed PMID: 3125607.

162: Fierke CA, Johnson KA, Benkovic SJ. Construction and evaluation of the
kinetic scheme associated with dihydrofolate reductase from Escherichia coli.
Biochemistry. 1987 Jun 30;26(13):4085-92. PubMed PMID: 3307916.

163: Fierke CA, Kuchta RD, Johnson KA, Benkovic SJ. Implications for enzymic
catalysis from free-energy reaction coordinate profiles. Cold Spring Harb Symp
Quant Biol. 1987;52:631-8. PubMed PMID: 3331348.

164: Mayer RJ, Chen JT, Taira K, Fierke CA, Benkovic SJ. Importance of a
hydrophobic residue in binding and catalysis by dihydrofolate reductase. Proc
Natl Acad Sci U S A. 1986 Oct;83(20):7718-20. PubMed PMID: 3463995; PubMed
Central PMCID: PMC386792.

165: Fierke CA, Jencks WP. Two functional domains of coenzyme A activate
catalysis by coenzyme A transferase. Pantetheine and adenosine 3'-phosphate
5'-diphosphate. J Biol Chem. 1986 Jun 15;261(17):7603-6. PubMed PMID: 3458707.

166: Chen JT, Mayer RJ, Fierke CA, Benkovic SJ. Site-specific mutagenesis of
dihydrofolate reductase from Escherichia coli. J Cell Biochem. 1985;29(2):73-82.
PubMed PMID: 3905833.





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