Inamdar, M. V., Kim, T., Chung, Y.-K., Was, A. M., Xiang, X., Wang, C.-W., Takayama, S., Lastoskie, C. M., T
homas, F. I.M., and Sastry, A.M., 2007, "Assessment of Sperm Chemokinesis with Exposure to Jelly Coats of Sea Urchin Eggs and Resact: A Microfluidic Experiment, and Numerical Study," The Journal of Experimental Biolog, v. 210(21), pp. 3805-3820.

Abstract


Specific peptides contained within the extracellular layer, or jelly coat, of a sea urchin egg have been hypothesized to play an important role in fertilization, though separate accounting of the effects of chemoattraction, chemokinesis, sperm agglomeration, and the other possible roles of the jelly coat, have not been reported. In the present study, we used a microfluidic device that allowed determination of the differences in the diffusion coefficients of
sperm of the purple sea urchin, Arbacia Punctulata subjected to two chemoattractants namely the jelly coat and resact. Our objectives were twofold: 1) to experimentally determine and compare the diffusion coefficients of Arbacia Punctulata spermatozoa in sea water, jelly
coat solution and resact solution; and 2) to determine the effect of sea urchin sperm diffusion coefficient and egg size on the sperm-egg collision frequency using stochastic simulations. Numerical values of the diffusion coefficients obtained by diffusing the spermatozoa in sea water, resact solution and jelly coat solution, were used to quantify the chemotactic effect. This allowed direct incorporation of known enlargements of the egg, and altered sperm diffusion
coefficients in the presence of chemoattractant, in the stochastic simulations. Simulation results showed that increase in diffusion coefficient values and egg diameter values increased the collision frequency. From the simulation results, we concluded that type of sperm, egg diameter and diffusion coefficient are significant factors in egg fertilization. Increasing the motility of sperm appears to be the prominent role of the jelly coat.